SISE p1 was further fractionated by DEAE cellulose ion exchange chromatography and four fractions were obtained by stepwise elution. The fraction eluted with Tris HCl buffer containing 0.2 mol of sodium chloride per liter had the highest activity. 用DEAE-纤维素离子交换层析对SISE-p1进行分离,生物活性研究表明用含0.2mol/L氯化钠的Tris-HCl缓冲液(pH7.0)洗脱的组分具有较强的生物活性。
Based on "moving overlapping separation range map" method, a novel method of optimizing multi-segment stepwise gradient elution conditions, "adjustable moving overlapping separation range map" method, was developed. 基于移动重叠分离图方法,又发展了一种新型的多台阶梯度分离条件的优化方法可调移动重叠分离图法。
By using H-600 resin adsorption and stepwise elution chromatography process for tea-polyphenols and caffeine separation, this process can solve the problem in conventional process in which caffeine must be eliminated with poisonous solvent. 采用H-600树脂吸附阶段洗脱层析法分离茶多酚***,解决了传统工艺中使用有毒溶剂脱除***的难题。
Stepwise elution adsorption chromatography separation for tea polyphenols and caffeine 阶段洗脱吸附层析法分离茶多酚***
LSD is selected as the medium. The feed-in solvent is 90% ethanol and the solvent of desorption is stepwise elution by 90% ethanol and water. 选LSD树脂为层析介质,料液溶剂为90%乙醇水溶液,用90%乙醇水溶液和纯水进行两阶逐次洗脱。